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Erlenmeyer Flasks

🛡️ DNase/RNase · DNA · Pyrogen · ATP-Free
✨ Sterile
Erlenmeyer flasks in PC and PETG, available in sizes from 125 mL to 5,000 mL, with flat or baffled bottoms and plug or vent caps. Seamless one-time forming eliminates weld seams and surface nodules, resulting in superior cell culture performance. 
  • SBS standard footprint enables direct bioreactor quick-connection.
  • Individually vacuum-packaged. 
  • PC flasks are autoclavable.
  • PETG rated to -70°C. 
  • 0.22 µm hydrophobic membrane on all vent cap versions.
SKU: EF1-CF125-V
  • Select a Erlenmeyer Flask Volume
  • Select Cap Type
  • Select an Erlenmeyer Flask Bottom Type
  • Select Erlenmeyer Material
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Price
118.45 118.45 (Tax excluded)

For Research Use Only
Return & Refund Policy
Description
Key Features
Specifications
Storage & Handling
FAQs
Product Description

Sterile Erlenmeyer flasks manufactured from medical-grade PETG or polycarbonate (PC) using seamless, one-time forming technology — eliminating surface nodules and weld seams that can disrupt cell growth and introduce contamination risk. Available in flat bottom and baffled bottom configurations with plug cap or vented cap options, from 125 mL to 5,000 mL.

Flask dimensions meet the international SBS standard for direct compatibility with standard bioreactor quick-connection systems — streamlining scale-up workflows from bench to bioreactor without additional adapters. Each flask is individually vacuum-packaged to maintain sterility until point of use.

Material Options:

PC
Polycarbonate
Autoclavable — 121°C, 15 PSI, 15–20 min Available in flat and baffled bottom 125 mL to 5,000 mL Plug cap and vent cap options
PETG
PETG
Rated to -70°C for cold storage Flat bottom only 125 mL to 1,000 mL Plug cap and vent cap options

Configuration Options:

Bottom Type
Flat Bottom
Standard flat base for bench use, magnetic stirring, and general culture applications. Available in both PC and PETG.
Bottom Type
Baffled Bottom
Internal baffles increase aeration and mixing efficiency during shaking culture — ideal for suspension culture, microbial fermentation, and high-density growth. PC only.
Cap Type
Plug Cap
Sealed closure for standard culture, storage, and transport applications requiring a fully closed system.
Cap Type
Vent Cap — 0.22 µm
Hydrophobic 0.22 µm membrane allows passive gas exchange while blocking microbial contamination — ideal for aerobic culture and CO₂ incubator applications.
Key Features & Benefits
  • Seamless one-time forming construction — eliminates weld seams and surface nodules that can disrupt cell attachment, cause cell damage, or introduce contamination
  • SBS standard dimensions — flask footprint meets international SBS standards for direct quick-connection compatibility with standard bioreactor systems — no additional adapters required
  • Individual vacuum packaging — each flask is individually vacuum-sealed to maintain sterility until point of use
  • Baffled bottom option — internal baffles significantly increase aeration and mixing efficiency during orbital shaking — maximizing oxygen transfer and cell density for suspension culture and microbial fermentation
  • Flat bottom option — suitable for magnetic stirring, standard bench culture, and general laboratory applications
  • 0.22 µm hydrophobic vent cap — membrane filter allows passive gas exchange while blocking microbial contamination — superior gas transfer for aerobic culture without opening the flask
  • Plug cap option — fully sealed closure for closed-system applications, transport, and storage
  • PC material — autoclavable — polycarbonate construction withstands standard autoclave sterilization at 121°C, 15 PSI for 15–20 minutes for re-sterilization workflows
  • PETG material — rated to -70°C — suitable for cold and ultra-low temperature storage applications
  • Medical-grade materials — manufactured from medical-grade PETG and PC for consistent, reproducible culture performance
  • DNase/RNase, Endotoxin, Human DNA, ATP, and Pyrogen-free — certified for sensitive cell culture and biological applications
  • Sterile — gamma irradiated, dose verification per ISO 11137, SAL 10⁻⁶
⚠️ PETG flasks are not autoclavable — do not heat-sterilize PETG. PC flasks only are autoclavable at 121°C, 15 PSI for 15–20 minutes.
Specifications
SpecificationDetail
Material OptionsPC (Polycarbonate) or PETG
ConstructionSeamless one-time forming — no weld seams or surface nodules
Bottom OptionsFlat Bottom or Baffled Bottom
Cap OptionsPlug Cap or Vent Cap (0.22 µm hydrophobic membrane)
Vent Cap Filter0.22 µm hydrophobic membrane — microbial barrier with gas exchange
Footprint Standard✦ SBS Standard — bioreactor compatible
PackagingIndividual vacuum packaging
PC — Available Sizes125 mL  |  250 mL  |  500 mL  |  1,000 mL  |  2,000 mL  |  3,000 mL  |  5,000 mL
PC — Bottom OptionsFlat Bottom and Baffled Bottom
PC — Autoclavable△ Yes — 121°C, 15 PSI, 15–20 minutes
PETG — Available Sizes125 mL  |  250 mL  |  500 mL  |  1,000 mL
PETG — Bottom OptionsFlat Bottom only
PETG — Temperature Rating❄ Rated to -70°C
PETG — Autoclavable⚠️ Not Autoclavable
Sterile✓ Yes — individually vacuum-packaged
Sterilization MethodGamma irradiation — dose verification per ISO 11137
Sterility Assurance LevelSAL 10⁻⁶
Quality SystemManufactured under ISO 13485
BiocompatibilityMeets USP Class VI requirements
RNase / DNase-free✓ Certified
Endotoxin-free✓ Certified
Human DNA-free✓ Certified
Pyrogen-free✓ Certified
ATP-free✓ Certified
Storage & Handling
  • Store in original vacuum packaging in a clean, dry environment away from direct sunlight and UV exposure
  • Maintain storage temperature between 15°C and 30°C (59°F – 86°F) prior to use
  • PETG flasks — suitable for storage at temperatures down to -70°C
  • PC flasks — autoclavable at 121°C, 15 PSI for 15–20 minutes for re-sterilization; allow to cool completely before use
  • Open vacuum packaging aseptically inside a biosafety cabinet at point of use
  • Do not use flasks from damaged or previously opened vacuum packaging
  • When using vent cap flasks in a shaking incubator, ensure cap is securely tightened to prevent liquid contact with the 0.22 µm membrane during agitation
  • Do not fill above 20–30% of nominal flask volume for shaking culture applications to ensure adequate gas exchange and mixing
  • Inspect flasks for cracks or stress marks before use — do not use damaged flasks
  • Dispose of used flasks in accordance with your institution's biosafety waste disposal guidelines
⚠️ PETG flasks are not autoclavable — autoclaving will permanently deform PETG material. PC flasks only may be autoclaved. Do not fill shaking culture flasks above 20–30% of nominal volume — overfilling reduces gas exchange and mixing efficiency.
Frequently Asked Questions
What is the advantage of seamless one-time forming construction?
Traditional Erlenmeyer flasks made from multiple pieces are assembled using weld seams or joining processes — these seams can create surface irregularities, micro-crevices, and nodules that disrupt cell growth, cause mechanical cell damage during agitation, and create sites where contamination can accumulate. Seamless one-time forming produces a uniform, smooth internal surface with no joints or irregularities — reducing cell damage, improving culture reproducibility, and simplifying cleaning and sterilization.
What does SBS standard compatibility mean?
The Society for Biomolecular Sciences (SBS) standard defines standardized footprint dimensions for laboratory vessels and equipment. Erlenmeyer flasks that meet SBS standards have base dimensions compatible with standard bioreactor quick-connection ports and automated liquid handling systems — allowing direct connection without additional adapters or custom fittings. This simplifies scale-up from flask culture to bioreactor and integrates with standard automated bioprocessing workflows.
When should I choose a baffled bottom vs flat bottom flask?
Choose a baffled bottom flask when your application requires enhanced aeration and mixing — such as suspension cell culture, microbial fermentation, and high-density growth workflows where oxygen transfer is critical. The internal baffles disrupt laminar flow during orbital shaking, dramatically increasing oxygen transfer rates and improving mixing uniformity. Flat bottom flasks are better suited for standard bench applications, magnetic stirring, and workflows where the baffles are not needed.
What is the difference between plug cap and vent cap?
The plug cap provides a fully sealed closure — suitable for sealed storage, transport, and culture applications where gas exchange is not required or where CO₂ balance is maintained by opening the cap periodically. The vent cap contains a 0.22 µm hydrophobic membrane that allows passive gas exchange — CO₂ in and O₂ out — while blocking microbial contamination. Use the vent cap for aerobic shaking culture and any application where continuous gas exchange is required without opening the flask.
Can PC Erlenmeyer flasks be autoclaved?
Yes — PC (polycarbonate) flasks are autoclavable at 121°C at 15 PSI for 15–20 minutes using standard laboratory autoclave protocol. Allow flasks to cool completely before handling after autoclaving. PETG flasks are not autoclavable and will permanently deform under autoclave conditions — all flasks are supplied pre-sterilized by gamma irradiation so autoclaving is only required for re-sterilization of PC flasks between uses.
Are Certificates of Analysis available?
Yes — Certificates of Analysis (COA) are available upon request for all Erlenmeyer flasks. Each lot is tested and certified RNase-, DNase-, Endotoxin-, Human DNA-, ATP-, and Pyrogen-free, manufactured under ISO 13485, and meets USP Class VI biocompatibility requirements. Contact us with your lot number for documentation.
Configuration & Packaging Reference — click to expand
Volume Material Bottom Cap Flasks/
Case
Autoclavable
PC — Flat Bottom
125 mLPCFlatPlug Cap24△ Yes
250 mLPCFlatPlug Cap12△ Yes
500 mLPCFlatPlug Cap12△ Yes
1,000 mLPCFlatPlug Cap6△ Yes
125 mLPCFlatVent Cap24△ Yes
250 mLPCFlatVent Cap12△ Yes
500 mLPCFlatVent Cap12△ Yes
1,000 mLPCFlatVent Cap6△ Yes
2,000 mLPCFlatVent Cap6△ Yes
3,000 mLPCFlatVent Cap4△ Yes
5,000 mLPCFlatVent Cap4△ Yes
PC — Baffled Bottom
125 mLPCBaffledPlug Cap24△ Yes
250 mLPCBaffledPlug Cap12△ Yes
500 mLPCBaffledPlug Cap12△ Yes
1,000 mLPCBaffledPlug Cap6△ Yes
125 mLPCBaffledVent Cap24△ Yes
250 mLPCBaffledVent Cap12△ Yes
500 mLPCBaffledVent Cap12△ Yes
1,000 mLPCBaffledVent Cap6△ Yes
2,000 mLPCBaffledVent Cap6△ Yes
3,000 mLPCBaffledVent Cap4△ Yes
PETG — Flat Bottom (❄ Rated to -70°C)
125 mLPETGFlatPlug Cap24⚠️ No
250 mLPETGFlatPlug Cap12⚠️ No
500 mLPETGFlatPlug Cap12⚠️ No
1,000 mLPETGFlatPlug Cap6⚠️ No
125 mLPETGFlatVent Cap24⚠️ No
250 mLPETGFlatVent Cap12⚠️ No
500 mLPETGFlatVent Cap12⚠️ No
1,000 mLPETGFlatVent Cap6⚠️ No
* PC (purple badges) = autoclavable at 121°C / 15 PSI / 15–20 min. PETG (blue badges) = not autoclavable, rated to -70°C. All flasks are sterile (SAL 10⁻⁶), individually vacuum-packaged, SBS standard footprint, with 0.22 µm hydrophobic membrane on vent cap versions.