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Technical Resources
Protocols, Specs & Application Notes
Lab techniques and application guides to help you get the most from your Kulture Scientific products and your cell culture work.
Cell Culture Protocols ▼
Passaging Adherent Cell Lines Cell Confluency Guide Cryopreservation Contamination Troubleshooting
Application Notes ▼
Balanced Salt Solutions
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Cell Culture Protocols
Step-by-step protocols and technique guides for mammalian cell culture workflows
Passaging & Subculture
T-flasks in a biosafety cabinet illustrationProtocol
Cell Culture
Standard Passaging Protocol for Adherent Cell Lines
Step-by-step protocol for passaging adherent mammalian cells: detachment, counting, and reseeding. Covers PBS wash rationale, trypsin neutralization, and best practices for maintaining healthy cultures.
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Confluency & Culture Assessment
Cell confluency reference illustration showing 25% to 100% confluencyGuide
Cell Culture
Cell Confluency Guide: How to Assess and Estimate Accurately
How to visually estimate confluency accurately, with common mistakes, morphology-specific considerations, and reference images to calibrate your eye. Includes guidance on when to passage based on confluency.
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Cryopreservation of Mammalian Cells
Passive cooling container for cryopreservation of mammalian cell linesProtocol
Cell Culture
Cryopreservation Protocol for Mammalian Cell Lines
Freezing and recovery of adherent and suspension cell lines. Covers serum formulation, DMSO concentration, passive cooling, and liquid nitrogen vapor-phase storage.
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Contamination & Troubleshooting
Cell culture contamination overview illustrationGuide
Cell Culture
Cell Culture Contamination Troubleshooting Guide
Identify bacteria, yeast, mold, and mycoplasma, respond fast, and find the source when contamination keeps coming back. Includes reference images, a step-by-step response plan, and a comparison of mycoplasma testing methods.
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More protocols coming soon Cell factory scale-up, decontamination and quarantine procedures, and pipetting best practices are in development. Check back or contact us if you need a protocol sooner.
Buffers & Reagents
Illustration of PBS, DPBS, HBSS, and EBSS reagent bottles Application Note
Cell Culture
Choosing the Right Balanced Salt Solution
PBS, DPBS, HBSS, and EBSS compared: verified formulations, sterilization methods, and when to use each.
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