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- Cell Factory 0.22uM Filter Assemblies
- Cell Factory and Accessories
Description
Key Features
Specifications
Storage & Handling
FAQs
Product Description
Sterile 0.22 µm vent filter assemblies for Cell Factory systems — providing sterile air and gas inlet and outlet filtration in closed cell culture and bioprocessing workflows. Available in butterfly and bag filter configurations with import and standard connection types to match your Cell Factory system setup. Each assembly is individually packaged sterile, ready to use from the bag.
The 0.22 µm membrane rating ensures complete retention of bacteria, fungi, and other microorganisms from air and gas streams entering and exiting the Cell Factory unit — maintaining the sterile integrity of the closed system throughout cell culture, media exchange, and harvest operations. Certified DNase/RNase-free and endotoxin-free for sensitive biological manufacturing applications including vaccine production, therapeutic protein expression, cell therapy, and gene therapy.
Filter Type Options
Filter Type
Butterfly Filter Assembly
Format: Compact butterfly-style vent filter
Best for: Standard vent port connections on Cell Factory units
Design: Low profile — minimizes dead volume on vent lines
Filter Type
Bag Filter Assembly
Format: Bag-style vent filter assembly
Best for: Higher flow vent applications and larger Cell Factory configurations
Design: Larger filter area for extended runs and higher gas flow rates
Key Features & Benefits
- 0.22 µm membrane rating — complete retention of bacteria, fungi, and microorganisms from air and gas streams entering and exiting the Cell Factory unit
- Sterile vent filtration — maintains closed system sterile integrity during cell culture, media exchange, perfusion, and harvest operations
- Butterfly filter option — compact low-profile design for standard vent port connections, minimizing dead volume on vent lines
- Bag filter option — larger filter area for higher gas flow rates and extended run applications in larger Cell Factory configurations
- Two connection types — import and standard to match your Cell Factory system configuration
- Pre-assembled and sterile — individually packaged sterile, ready to use directly from the bag — no assembly or sterilization required
- Gamma irradiation sterilized — dose verification per ISO 11137, SAL 10⁻⁶
- DNase/RNase-free and endotoxin-free — certified for vaccine production, therapeutic protein expression, cell therapy, and gene therapy manufacturing workflows
- Single-use design — eliminates cleaning validation and reduces cross-contamination risk between production runs
Specifications
| Specification | Detail |
|---|---|
| Filter Type | Butterfly filter assembly | Bag filter assembly |
| Pore Size | 0.22 µm |
| Application | Sterile vent filtration — air and gas inlet and outlet |
| Connection Type | Import | Standard |
| Sterile | ✨ Yes — individually packaged sterile |
| Sterilization Method | Gamma irradiation — dose verification per ISO 11137 |
| Sterility Assurance Level | SAL 10⁻⁶ |
| DNase / RNase-free | ✓ Certified |
| Endotoxin-free | ✓ Certified |
| Single-Use | ✓ Single-use recommended |
| Packaging | 1 assembly per sterile bag |
| Compatible With | Cell Factory 1, 2, 4, 10, and 40-layer systems |
| Applications | Vaccines | Therapeutic proteins | Cell therapy | Gene therapy |
Storage & Handling
- Store in original sterile packaging in a clean, dry environment away from direct sunlight and UV exposure
- Maintain storage temperature between 15°C and 30°C prior to use
- Do not use assemblies from damaged or previously opened packaging
- Open sterile packaging aseptically inside a biosafety cabinet at point of use
- Inspect filter housing and connections for damage before connecting to the Cell Factory vent port
- Ensure filter assembly is correctly oriented — verify inlet and outlet direction before connecting to the vent line
- Do not allow filter membrane to become wet during use — liquid blockage will restrict gas flow and compromise vent filtration performance
- Single-use only — do not reuse filter assemblies between runs
- Replace filter assemblies if resistance to airflow increases noticeably during a run — this may indicate membrane blockage
- Dispose of used assemblies per your institution's biosafety and single-use material disposal guidelines
Frequently Asked Questions
What is the purpose of the vent filter on a Cell Factory system?
Cell Factory systems require sterile gas exchange during cell culture, media addition, and harvest operations. Without a vent filter, ambient air entering or exiting the system through the vent port would introduce airborne microorganisms and contaminate the cell culture. The 0.22 µm vent filter sterilizes all air and gas passing through the vent port — maintaining closed system sterile integrity throughout the entire production run.
When should I choose the butterfly filter vs the bag filter?
The butterfly filter is the standard choice for most Cell Factory vent applications — its compact low-profile design minimizes dead volume and suits standard vent port connections. The bag filter is preferred for larger Cell Factory configurations (10 and 40-layer) where higher gas flow rates are required during rapid media exchange, perfusion, or harvest, as the larger filter area reduces back pressure and extends filter performance over longer runs.
What happens if the vent filter membrane gets wet?
If the hydrophilic filter membrane becomes saturated with liquid, it will block gas flow through the vent — preventing the Cell Factory from breathing during media exchange and potentially creating pressure differentials that can damage the unit or compromise sterility. Always ensure the filter is correctly oriented and that liquid does not reach the filter during media addition or harvest operations. Replace the filter immediately if wetout occurs.
Are these filter assemblies compatible with all Cell Factory system sizes?
Yes — these vent filter assemblies are compatible with Cell Factory 1, 2, 4, 10, and 40-layer systems. Select the appropriate filter type (butterfly or bag) and connection type to match your specific Cell Factory system configuration and run requirements.
Are Certificates of Analysis available?
Yes — Certificates of Analysis (COA) are available upon request. Each lot is certified DNase/RNase-free and endotoxin-free, gamma irradiated per ISO 11137, SAL 10⁻⁶. Contact us with your lot number for documentation.
Description
Key Features
Specifications
Storage & Handling
FAQs
Product Description
Sterile 0.22 µm vent filter assemblies for Cell Factory systems — providing sterile air and gas inlet and outlet filtration in closed cell culture and bioprocessing workflows. Available in butterfly and bag filter configurations with import and standard connection types to match your Cell Factory system setup. Each assembly is individually packaged sterile, ready to use from the bag.
The 0.22 µm membrane rating ensures complete retention of bacteria, fungi, and other microorganisms from air and gas streams entering and exiting the Cell Factory unit — maintaining the sterile integrity of the closed system throughout cell culture, media exchange, and harvest operations. Certified DNase/RNase-free and endotoxin-free for sensitive biological manufacturing applications including vaccine production, therapeutic protein expression, cell therapy, and gene therapy.
Filter Type Options
Filter Type
Butterfly Filter Assembly
Format: Compact butterfly-style vent filter
Best for: Standard vent port connections on Cell Factory units
Design: Low profile — minimizes dead volume on vent lines
Filter Type
Bag Filter Assembly
Format: Bag-style vent filter assembly
Best for: Higher flow vent applications and larger Cell Factory configurations
Design: Larger filter area for extended runs and higher gas flow rates
Key Features & Benefits
- 0.22 µm membrane rating — complete retention of bacteria, fungi, and microorganisms from air and gas streams entering and exiting the Cell Factory unit
- Sterile vent filtration — maintains closed system sterile integrity during cell culture, media exchange, perfusion, and harvest operations
- Butterfly filter option — compact low-profile design for standard vent port connections, minimizing dead volume on vent lines
- Bag filter option — larger filter area for higher gas flow rates and extended run applications in larger Cell Factory configurations
- Two connection types — import and standard to match your Cell Factory system configuration
- Pre-assembled and sterile — individually packaged sterile, ready to use directly from the bag — no assembly or sterilization required
- Gamma irradiation sterilized — dose verification per ISO 11137, SAL 10⁻⁶
- DNase/RNase-free and endotoxin-free — certified for vaccine production, therapeutic protein expression, cell therapy, and gene therapy manufacturing workflows
- Single-use design — eliminates cleaning validation and reduces cross-contamination risk between production runs
Specifications
| Specification | Detail |
|---|---|
| Filter Type | Butterfly filter assembly | Bag filter assembly |
| Pore Size | 0.22 µm |
| Application | Sterile vent filtration — air and gas inlet and outlet |
| Connection Type | Import | Standard |
| Sterile | ✨ Yes — individually packaged sterile |
| Sterilization Method | Gamma irradiation — dose verification per ISO 11137 |
| Sterility Assurance Level | SAL 10⁻⁶ |
| DNase / RNase-free | ✓ Certified |
| Endotoxin-free | ✓ Certified |
| Single-Use | ✓ Single-use recommended |
| Packaging | 1 assembly per sterile bag |
| Compatible With | Cell Factory 1, 2, 4, 10, and 40-layer systems |
| Applications | Vaccines | Therapeutic proteins | Cell therapy | Gene therapy |
Storage & Handling
- Store in original sterile packaging in a clean, dry environment away from direct sunlight and UV exposure
- Maintain storage temperature between 15°C and 30°C prior to use
- Do not use assemblies from damaged or previously opened packaging
- Open sterile packaging aseptically inside a biosafety cabinet at point of use
- Inspect filter housing and connections for damage before connecting to the Cell Factory vent port
- Ensure filter assembly is correctly oriented — verify inlet and outlet direction before connecting to the vent line
- Do not allow filter membrane to become wet during use — liquid blockage will restrict gas flow and compromise vent filtration performance
- Single-use only — do not reuse filter assemblies between runs
- Replace filter assemblies if resistance to airflow increases noticeably during a run — this may indicate membrane blockage
- Dispose of used assemblies per your institution's biosafety and single-use material disposal guidelines
Frequently Asked Questions
What is the purpose of the vent filter on a Cell Factory system?
Cell Factory systems require sterile gas exchange during cell culture, media addition, and harvest operations. Without a vent filter, ambient air entering or exiting the system through the vent port would introduce airborne microorganisms and contaminate the cell culture. The 0.22 µm vent filter sterilizes all air and gas passing through the vent port — maintaining closed system sterile integrity throughout the entire production run.
When should I choose the butterfly filter vs the bag filter?
The butterfly filter is the standard choice for most Cell Factory vent applications — its compact low-profile design minimizes dead volume and suits standard vent port connections. The bag filter is preferred for larger Cell Factory configurations (10 and 40-layer) where higher gas flow rates are required during rapid media exchange, perfusion, or harvest, as the larger filter area reduces back pressure and extends filter performance over longer runs.
What happens if the vent filter membrane gets wet?
If the hydrophilic filter membrane becomes saturated with liquid, it will block gas flow through the vent — preventing the Cell Factory from breathing during media exchange and potentially creating pressure differentials that can damage the unit or compromise sterility. Always ensure the filter is correctly oriented and that liquid does not reach the filter during media addition or harvest operations. Replace the filter immediately if wetout occurs.
Are these filter assemblies compatible with all Cell Factory system sizes?
Yes — these vent filter assemblies are compatible with Cell Factory 1, 2, 4, 10, and 40-layer systems. Select the appropriate filter type (butterfly or bag) and connection type to match your specific Cell Factory system configuration and run requirements.
Are Certificates of Analysis available?
Yes — Certificates of Analysis (COA) are available upon request. Each lot is certified DNase/RNase-free and endotoxin-free, gamma irradiated per ISO 11137, SAL 10⁻⁶. Contact us with your lot number for documentation.
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