- All products
- Kultureware
- Cell Culture Flasks
- Cell Culture Flasks
- Cell Culture Flasks
Description
Key Features
Specifications
Storage & Handling
FAQs
Product Description
Designed for standard and large-scale cell culture workflows, these tissue culture flasks feature an angled neck with an extra-wide opening for unobstructed pipette and scraper access to the growth surface — reducing cell loss during harvest and improving recovery consistency. The raised outer base provides a stable stacking profile for incubator and bench storage.
Available in TC-Treated and Suspension configurations to match your specific culture workflow:
TC-Treated
Adherent Cell Culture
"The Welcome Mat"
Hydrophilic surface draws adherent cells in, encouraging attachment and healthy monolayer formation. Ideal for primary cells, adherent cell lines, and standard tissue culture workflows.
Suspension
Non-Adherent Cell Culture
"The Non-Stick Surface"
Smooth, non-treated interior stays deliberately inert — keeping suspension cells freely floating without unwanted surface interaction. Ideal for suspension cultures, bacterial growth, and non-adherent cell lines.
Key Features & Benefits
- Angled ergonomic neck — provides comfortable, direct access to the growth surface, reducing wrist strain during pipetting and cell scraping
- Extra-wide neck opening — accommodates standard cell scrapers and pipettes without awkward maneuvering for maximum cell recovery during harvest
- Raised outer bottom edge — creates a stable, level base for secure stacking in the incubator or on the bench without slipping or tipping
- TC-Treated surface — hydrophilic treatment promotes cell attachment and monolayer formation for adherent cell lines
- Suspension surface — smooth, non-treated interior keeps non-adherent cells freely floating without unwanted surface interaction
- Vented cap option — permeable membrane allows passive CO₂ gas exchange for consistent incubator culture without opening the flask
- Plug cap option — maintains a fully sealed environment for closed-system applications and short-term transport
- Precision graduation markings — clear volume indicators for quick visual confirmation during media changes
- DNase/RNase, DNA, Pyrogen, and ATP-free — certified safe for sensitive cell culture applications
- Sterile, individually wrapped — sterility maintained until point of use
⚠️ Single-use only — not autoclavable. Do not attempt to re-sterilize or reuse. Reuse may compromise sterility and introduce contamination risk to your culture.
Specifications
| Specification | Detail |
|---|---|
| Material | Virgin polystyrene |
| Sterilization Method | Gamma irradiation — dose verification per ISO 11137 |
| Sterility Assurance Level | SAL 10⁻⁶ |
| Available Sizes | 25 cm² | 75 cm² | 175 cm² | 225 cm² |
| Surface Treatment | TC-Treated (adherent) or Suspension (non-treated) |
| Cap Options | Plug Cap or Vented Cap |
| Neck Design | Angled ergonomic, extra-wide opening |
| Base Design | Raised outer edge for stable stacking |
| Graduation Marks | ✓ Yes |
| DNase / RNase-free | ✓ Certified |
| DNA-free | ✓ Certified |
| Pyrogen-free | ✓ Certified |
| ATP-free | ✓ Certified |
| Sterile | ✓ Yes — individually wrapped |
| Autoclavable | ⚠️ Not Autoclavable |
| Single-Use | ⚠️ Single-Use Only |
Storage & Handling
- Store in original sealed packaging in a clean, dry environment away from direct sunlight and UV exposure
- Maintain storage temperature between 15°C and 30°C (59°F – 86°F)
- Keep away from excessive humidity
- Do not use flasks from damaged or previously opened packaging
- Handle with gloves using standard aseptic technique inside a biosafety cabinet
- Inspect packaging integrity before opening — do not use if seal is compromised
- Single-use only — do not reuse between passages or experiments
- Dispose of used flasks in accordance with your institution's biosafety waste disposal guidelines
⚠️ Not autoclavable — the polystyrene material will deform under autoclave conditions. Autoclaving will also compromise the TC-treated surface treatment. Flasks are pre-sterilized by gamma irradiation and certified contaminant-free from the factory.
Frequently Asked Questions
What is the difference between TC-Treated and Suspension flasks?
TC-Treated flasks have a hydrophilic surface that promotes cell attachment — think of it as a cellular welcome mat. They are designed for adherent cell lines that need to grip and spread across the growth surface to form a healthy monolayer. Suspension flasks have a smooth, non-treated interior that stays deliberately inert, keeping cells freely floating without unwanted surface interaction. Use suspension flasks for bacterial cultures, non-adherent cell lines, and applications where you do not want cells attaching to the flask wall.
When should I use a vented cap vs. a plug cap?
Use a vented cap when growing cells in a CO₂ incubator — the permeable membrane allows passive gas exchange to maintain consistent CO₂ and pH balance without opening the flask. Use a plug cap for closed-system applications, short-term transport, or when you need a fully sealed environment. If you are unsure, vented caps are the standard choice for most routine cell culture in a CO₂ incubator.
Why does the angled neck design matter?
The angled ergonomic neck provides direct, unobstructed access to the growth surface — especially important when using a cell scraper for cell harvesting. Standard straight-neck flasks can require awkward wrist angles that make complete scraping difficult and leave cells behind. The angled design combined with the extra-wide opening means your scraper or pipette reaches the entire growth surface comfortably, minimizing cell loss and reducing hand fatigue during repeated passages.
Can these flasks be autoclaved or reused?
No. These flasks are single-use only and must not be autoclaved or reused. The polystyrene material is not heat-resistant and will deform under autoclave conditions. Attempting to re-sterilize used flasks also compromises the TC-treated surface and sterility assurance. Each flask is gamma-irradiated and individually wrapped at the factory to ensure sterility at point of use.
What flask size should I use for my application?
Flask size selection depends on the scale of your culture. The 25 cm² flask is ideal for small-scale cultures, initial seeding, and cell line maintenance. The 75 cm² is the most common choice for routine passaging and general cell culture. The 175 cm² and 225 cm² are best for scale-up workflows, protein production, virus propagation, and applications requiring larger cell yields. As a general rule, select the smallest flask that meets your yield requirements to minimize reagent use.
Are Certificates of Analysis available?
Yes — Certificates of Analysis (COA) are available upon request for all tissue culture flasks. Each lot is tested and certified DNase/RNase-, DNA-, Pyrogen-, and ATP-free. Contact us with your lot number and we will provide the documentation for your records or regulatory compliance requirements.
Description
Key Features
Specifications
Storage & Handling
FAQs
Product Description
Designed for standard and large-scale cell culture workflows, these tissue culture flasks feature an angled neck with an extra-wide opening for unobstructed pipette and scraper access to the growth surface — reducing cell loss during harvest and improving recovery consistency. The raised outer base provides a stable stacking profile for incubator and bench storage.
Available in TC-Treated and Suspension configurations to match your specific culture workflow:
TC-Treated
Adherent Cell Culture
"The Welcome Mat"
Hydrophilic surface draws adherent cells in, encouraging attachment and healthy monolayer formation. Ideal for primary cells, adherent cell lines, and standard tissue culture workflows.
Suspension
Non-Adherent Cell Culture
"The Non-Stick Surface"
Smooth, non-treated interior stays deliberately inert — keeping suspension cells freely floating without unwanted surface interaction. Ideal for suspension cultures, bacterial growth, and non-adherent cell lines.
Key Features & Benefits
- Angled ergonomic neck — provides comfortable, direct access to the growth surface, reducing wrist strain during pipetting and cell scraping
- Extra-wide neck opening — accommodates standard cell scrapers and pipettes without awkward maneuvering for maximum cell recovery during harvest
- Raised outer bottom edge — creates a stable, level base for secure stacking in the incubator or on the bench without slipping or tipping
- TC-Treated surface — hydrophilic treatment promotes cell attachment and monolayer formation for adherent cell lines
- Suspension surface — smooth, non-treated interior keeps non-adherent cells freely floating without unwanted surface interaction
- Vented cap option — permeable membrane allows passive CO₂ gas exchange for consistent incubator culture without opening the flask
- Plug cap option — maintains a fully sealed environment for closed-system applications and short-term transport
- Precision graduation markings — clear volume indicators for quick visual confirmation during media changes
- DNase/RNase, DNA, Pyrogen, and ATP-free — certified safe for sensitive cell culture applications
- Sterile, individually wrapped — sterility maintained until point of use
⚠️ Single-use only — not autoclavable. Do not attempt to re-sterilize or reuse. Reuse may compromise sterility and introduce contamination risk to your culture.
Specifications
| Specification | Detail |
|---|---|
| Material | Virgin polystyrene |
| Sterilization Method | Gamma irradiation — dose verification per ISO 11137 |
| Sterility Assurance Level | SAL 10⁻⁶ |
| Available Sizes | 25 cm² | 75 cm² | 175 cm² | 225 cm² |
| Surface Treatment | TC-Treated (adherent) or Suspension (non-treated) |
| Cap Options | Plug Cap or Vented Cap |
| Neck Design | Angled ergonomic, extra-wide opening |
| Base Design | Raised outer edge for stable stacking |
| Graduation Marks | ✓ Yes |
| DNase / RNase-free | ✓ Certified |
| DNA-free | ✓ Certified |
| Pyrogen-free | ✓ Certified |
| ATP-free | ✓ Certified |
| Sterile | ✓ Yes — individually wrapped |
| Autoclavable | ⚠️ Not Autoclavable |
| Single-Use | ⚠️ Single-Use Only |
Storage & Handling
- Store in original sealed packaging in a clean, dry environment away from direct sunlight and UV exposure
- Maintain storage temperature between 15°C and 30°C (59°F – 86°F)
- Keep away from excessive humidity
- Do not use flasks from damaged or previously opened packaging
- Handle with gloves using standard aseptic technique inside a biosafety cabinet
- Inspect packaging integrity before opening — do not use if seal is compromised
- Single-use only — do not reuse between passages or experiments
- Dispose of used flasks in accordance with your institution's biosafety waste disposal guidelines
⚠️ Not autoclavable — the polystyrene material will deform under autoclave conditions. Autoclaving will also compromise the TC-treated surface treatment. Flasks are pre-sterilized by gamma irradiation and certified contaminant-free from the factory.
Frequently Asked Questions
What is the difference between TC-Treated and Suspension flasks?
TC-Treated flasks have a hydrophilic surface that promotes cell attachment — think of it as a cellular welcome mat. They are designed for adherent cell lines that need to grip and spread across the growth surface to form a healthy monolayer. Suspension flasks have a smooth, non-treated interior that stays deliberately inert, keeping cells freely floating without unwanted surface interaction. Use suspension flasks for bacterial cultures, non-adherent cell lines, and applications where you do not want cells attaching to the flask wall.
When should I use a vented cap vs. a plug cap?
Use a vented cap when growing cells in a CO₂ incubator — the permeable membrane allows passive gas exchange to maintain consistent CO₂ and pH balance without opening the flask. Use a plug cap for closed-system applications, short-term transport, or when you need a fully sealed environment. If you are unsure, vented caps are the standard choice for most routine cell culture in a CO₂ incubator.
Why does the angled neck design matter?
The angled ergonomic neck provides direct, unobstructed access to the growth surface — especially important when using a cell scraper for cell harvesting. Standard straight-neck flasks can require awkward wrist angles that make complete scraping difficult and leave cells behind. The angled design combined with the extra-wide opening means your scraper or pipette reaches the entire growth surface comfortably, minimizing cell loss and reducing hand fatigue during repeated passages.
Can these flasks be autoclaved or reused?
No. These flasks are single-use only and must not be autoclaved or reused. The polystyrene material is not heat-resistant and will deform under autoclave conditions. Attempting to re-sterilize used flasks also compromises the TC-treated surface and sterility assurance. Each flask is gamma-irradiated and individually wrapped at the factory to ensure sterility at point of use.
What flask size should I use for my application?
Flask size selection depends on the scale of your culture. The 25 cm² flask is ideal for small-scale cultures, initial seeding, and cell line maintenance. The 75 cm² is the most common choice for routine passaging and general cell culture. The 175 cm² and 225 cm² are best for scale-up workflows, protein production, virus propagation, and applications requiring larger cell yields. As a general rule, select the smallest flask that meets your yield requirements to minimize reagent use.
Are Certificates of Analysis available?
Yes — Certificates of Analysis (COA) are available upon request for all tissue culture flasks. Each lot is tested and certified DNase/RNase-, DNA-, Pyrogen-, and ATP-free. Contact us with your lot number and we will provide the documentation for your records or regulatory compliance requirements.
Growth Area & Working Volume Reference — click to expand
▼
| Growth Area | Flask Volume (mL) |
Recommended Working Volume (mL) |
Max Working Volume (mL) |
Recommended Dissociation Media (mL) |
|---|---|---|---|---|
| 25 cm² | 50 | 5 – 7.5 | 19 | 0.5 – 1 |
| 75 cm² | 250 | 15 – 22.5 | 55 | 2 – 4 |
| 175 cm² | 600 | 35 – 52.5 | 150 | 3 – 6 |
| 225 cm² | 900 | 60 – 90 | 310 | 5 – 10 |
25 cm²
50 mL flask
Recommended Vol.
5 – 7.5 mL
Max Volume
19 mL
Dissociation Media
0.5 – 1 mL
75 cm²
250 mL flask
Recommended Vol.
15 – 22.5 mL
Max Volume
55 mL
Dissociation Media
2 – 4 mL
175 cm²
600 mL flask
Recommended Vol.
35 – 52.5 mL
Max Volume
150 mL
Dissociation Media
3 – 6 mL
225 cm²
900 mL flask
Recommended Vol.
60 – 90 mL
Max Volume
310 mL
Dissociation Media
5 – 10 mL
* Recommended working volumes are based on standard seeding densities. Adjust as needed for your specific cell line and protocol. Dissociation media volumes are guidelines for trypsin or equivalent enzymatic dissociation.
Growth Area & Working Volume Reference — click to expand
▼
| Growth Area | Flask Volume (mL) |
Recommended Working Volume (mL) |
Max Working Volume (mL) |
Recommended Dissociation Media (mL) |
|---|---|---|---|---|
| 25 cm² | 50 | 5 – 7.5 | 19 | 0.5 – 1 |
| 75 cm² | 250 | 15 – 22.5 | 55 | 2 – 4 |
| 175 cm² | 600 | 35 – 52.5 | 150 | 3 – 6 |
| 225 cm² | 900 | 60 – 90 | 310 | 5 – 10 |
25 cm²
50 mL flask
Recommended Vol.
5 – 7.5 mL
Max Volume
19 mL
Dissociation Media
0.5 – 1 mL
75 cm²
250 mL flask
Recommended Vol.
15 – 22.5 mL
Max Volume
55 mL
Dissociation Media
2 – 4 mL
175 cm²
600 mL flask
Recommended Vol.
35 – 52.5 mL
Max Volume
150 mL
Dissociation Media
3 – 6 mL
225 cm²
900 mL flask
Recommended Vol.
60 – 90 mL
Max Volume
310 mL
Dissociation Media
5 – 10 mL
* Recommended working volumes are based on standard seeding densities. Adjust as needed for your specific cell line and protocol. Dissociation media volumes are guidelines for trypsin or equivalent enzymatic dissociation.
Frequently Bought Together
These other products might interest you