- All products
- PCR
- 96-well PCR Plates, Half-Skirted
- PCR
Description
Key Features
Specifications
Storage & Handling
FAQs
Product Description
❖ ABI Compatible — Applied Biosystems Instrument Fit
Half-skirted plate geometry is compatible with Applied Biosystems thermal cyclers and qPCR instruments including ABI 7500, 7900HT, and QuantStudio series, as well as a broad range of other instruments that accept the half-skirt format.
Half-skirted 96-well polypropylene qPCR plates in 0.1 mL and 0.2 mL well volumes — designed for Applied Biosystems thermal cyclers and other instruments accepting the half-skirt plate format. The half-skirt provides two raised side walls that support the plate during robotic handling and loading while maintaining the lower profile required by ABI and compatible instrument blocks. Available in clear and white wells with black printed or molded alphanumeric markings.
Ultra-thin well walls ensure optimal and uniform heat transfer for consistent thermal cycling performance. Raised well rims facilitate secure sealing across pressure-sensitive films, adhesive seals, and heat-sealing technologies. The seal between the tube opening and cap is optimized to minimize evaporation without causing spillage on opening. Non-sterile. Certified DNase/RNase-free and endotoxin-free. 10 plates per box, 100 plates per case.
Well Color Options
Well Color
Clear Wells
Transparent wells for visual sample inspection and broad compatibility with SYBR Green and probe-based detection methods.
Well Color
White Wells
White well walls reflect emitted fluorescence toward the detector — improving signal sensitivity in low-template and probe-based qPCR applications.
Plate Labeling Options
Labeling Style
Black Print
Alphanumeric identifiers printed in black ink on the plate frame. Available for 0.2 mL clear wells only.
Labeling Style
Molded
Alphanumeric identifiers permanently formed into the plate frame — cannot fade or wear off. Available for all color and volume combinations.
⚠️ Note — black print labeling is available for 0.2 mL clear wells only. All 0.1 mL variants and white wells are available with molded labeling only.
Applications
qPCR / Real-Time PCR
End-Point PCR
Gene Expression
SNP Genotyping
Pathogen Detection
Viral Load Quantification
Copy Number Variation
Key Features & Benefits
- Half-skirted format — ABI compatible — two raised side walls support the plate during robotic handling and instrument loading while maintaining the lower profile required by Applied Biosystems and compatible instruments
- Broad instrument compatibility — fits Applied Biosystems thermal cyclers including ABI 7500, 7900HT, and QuantStudio series, as well as other instruments accepting the half-skirt format
- Two well volumes — 0.1 mL for instruments with 0.1 mL block format and 0.2 mL for standard thermal cycler blocks
- Clear wells — transparent polypropylene wells for visual inspection and SYBR Green and probe-based detection compatibility
- White wells — white well surface reflects emitted fluorescence toward the detector for improved signal sensitivity in low-template applications
- Black print labeling (0.2 mL clear only) — high-contrast alphanumeric identifiers printed on the plate frame
- Molded labeling — permanently formed alphanumeric identifiers that cannot fade or wear off regardless of handling conditions
- Ultra-thin well walls — optimal and uniform heat transfer across all 96 wells for consistent thermal cycling performance
- Raised well rims — secure sealing with pressure-sensitive films, adhesive seals, and heat-sealing technologies
- Optimized seal design — tube wall thickness minimizes evaporation without causing spillage on opening
- DNase/RNase-free and endotoxin-free — certified for sensitive nucleic acid applications
Specifications
| Specification | Detail |
|---|---|
| Material | Polypropylene |
| Well Count | 96 wells |
| Skirt Style | Half-skirted |
| Instrument Compatibility | ❖ ABI compatible — Applied Biosystems and other instruments |
| Well Volumes | 0.1 mL | 0.2 mL |
| Well Color Options | Clear | White |
| Plate Labeling | Black print (0.2 mL clear only) | Molded (all variants) |
| Plate Markings | Alphanumeric — A–H rows, 1–12 columns |
| Sealing Compatibility | Pressure-sensitive films | Adhesive seals | Heat-sealing |
| Sterile | ⚠️ Non-Sterile |
| DNase / RNase-free | ✓ Certified |
| Endotoxin-free | ✓ Certified |
| Packaging | 10 plates per box | 100 plates per case |
Storage & Handling
- Store in original packaging in a clean, dry environment away from direct sunlight and UV exposure
- Maintain storage temperature between 15°C and 30°C prior to use
- Handle plates by the edges to avoid contaminating well surfaces with skin oils and RNases
- Use powder-free gloves when handling plates for sensitive RNA applications
- Load samples using a multichannel pipette or automated liquid handler to maintain well-to-well consistency
- Seal plates immediately after sample loading to prevent evaporation — verify seal integrity before placing in the thermal cycler
- Centrifuge briefly at low speed after sealing to collect sample at the well base and remove air bubbles
- Verify plate is compatible with your specific instrument model before use — confirm half-skirt format is accepted
- Dispose of used plates per your institution's biosafety and waste disposal guidelines
Frequently Asked Questions
What is a half-skirted plate and why does it matter?
A half-skirted plate has two raised side walls — typically along the long sides of the plate — that extend below the well base. These partial skirt walls provide support for robotic grippers and plate handlers during automated loading, while keeping the overall plate height low enough to fit the block format of ABI and other instruments that cannot accommodate a fully skirted plate. The half-skirt balances automation compatibility with instrument fit.
Which ABI instruments are these plates compatible with?
These half-skirted plates are compatible with Applied Biosystems thermal cyclers and qPCR instruments including the ABI 7500, 7900HT, and QuantStudio series that accept half-skirt plate formats. Always verify the recommended plate format in your specific instrument's documentation before ordering in bulk, as plate requirements can vary between ABI instrument models.
Why is black print labeling only available for 0.2 mL clear wells?
The available SKU combinations reflect what is stocked from the manufacturer. Black print labeling is offered on the 0.2 mL clear variant only. All other color and volume combinations — including all 0.1 mL variants and white well plates — are available with molded labeling, which is permanently formed into the plate frame and provides durable identification regardless of handling conditions.
When should I choose clear vs white wells?
Clear wells are the standard choice for most qPCR applications and are compatible with SYBR Green, probe-based, and multiplex detection methods. White wells are preferred for applications requiring maximum fluorescence sensitivity — the white surface reflects emitted light toward the detector, improving signal-to-noise ratio in low-template copy number assays and probe-based applications.
Are Certificates of Analysis available?
Yes — Certificates of Analysis (COA) are available upon request. Each lot is certified DNase/RNase-free and endotoxin-free. Contact us with your lot number for documentation.
Description
Key Features
Specifications
Storage & Handling
FAQs
Product Description
❖ ABI Compatible — Applied Biosystems Instrument Fit
Half-skirted plate geometry is compatible with Applied Biosystems thermal cyclers and qPCR instruments including ABI 7500, 7900HT, and QuantStudio series, as well as a broad range of other instruments that accept the half-skirt format.
Half-skirted 96-well polypropylene qPCR plates in 0.1 mL and 0.2 mL well volumes — designed for Applied Biosystems thermal cyclers and other instruments accepting the half-skirt plate format. The half-skirt provides two raised side walls that support the plate during robotic handling and loading while maintaining the lower profile required by ABI and compatible instrument blocks. Available in clear and white wells with black printed or molded alphanumeric markings.
Ultra-thin well walls ensure optimal and uniform heat transfer for consistent thermal cycling performance. Raised well rims facilitate secure sealing across pressure-sensitive films, adhesive seals, and heat-sealing technologies. The seal between the tube opening and cap is optimized to minimize evaporation without causing spillage on opening. Non-sterile. Certified DNase/RNase-free and endotoxin-free. 10 plates per box, 100 plates per case.
Well Color Options
Well Color
Clear Wells
Transparent wells for visual sample inspection and broad compatibility with SYBR Green and probe-based detection methods.
Well Color
White Wells
White well walls reflect emitted fluorescence toward the detector — improving signal sensitivity in low-template and probe-based qPCR applications.
Plate Labeling Options
Labeling Style
Black Print
Alphanumeric identifiers printed in black ink on the plate frame. Available for 0.2 mL clear wells only.
Labeling Style
Molded
Alphanumeric identifiers permanently formed into the plate frame — cannot fade or wear off. Available for all color and volume combinations.
⚠️ Note — black print labeling is available for 0.2 mL clear wells only. All 0.1 mL variants and white wells are available with molded labeling only.
Applications
qPCR / Real-Time PCR
End-Point PCR
Gene Expression
SNP Genotyping
Pathogen Detection
Viral Load Quantification
Copy Number Variation
Key Features & Benefits
- Half-skirted format — ABI compatible — two raised side walls support the plate during robotic handling and instrument loading while maintaining the lower profile required by Applied Biosystems and compatible instruments
- Broad instrument compatibility — fits Applied Biosystems thermal cyclers including ABI 7500, 7900HT, and QuantStudio series, as well as other instruments accepting the half-skirt format
- Two well volumes — 0.1 mL for instruments with 0.1 mL block format and 0.2 mL for standard thermal cycler blocks
- Clear wells — transparent polypropylene wells for visual inspection and SYBR Green and probe-based detection compatibility
- White wells — white well surface reflects emitted fluorescence toward the detector for improved signal sensitivity in low-template applications
- Black print labeling (0.2 mL clear only) — high-contrast alphanumeric identifiers printed on the plate frame
- Molded labeling — permanently formed alphanumeric identifiers that cannot fade or wear off regardless of handling conditions
- Ultra-thin well walls — optimal and uniform heat transfer across all 96 wells for consistent thermal cycling performance
- Raised well rims — secure sealing with pressure-sensitive films, adhesive seals, and heat-sealing technologies
- Optimized seal design — tube wall thickness minimizes evaporation without causing spillage on opening
- DNase/RNase-free and endotoxin-free — certified for sensitive nucleic acid applications
Specifications
| Specification | Detail |
|---|---|
| Material | Polypropylene |
| Well Count | 96 wells |
| Skirt Style | Half-skirted |
| Instrument Compatibility | ❖ ABI compatible — Applied Biosystems and other instruments |
| Well Volumes | 0.1 mL | 0.2 mL |
| Well Color Options | Clear | White |
| Plate Labeling | Black print (0.2 mL clear only) | Molded (all variants) |
| Plate Markings | Alphanumeric — A–H rows, 1–12 columns |
| Sealing Compatibility | Pressure-sensitive films | Adhesive seals | Heat-sealing |
| Sterile | ⚠️ Non-Sterile |
| DNase / RNase-free | ✓ Certified |
| Endotoxin-free | ✓ Certified |
| Packaging | 10 plates per box | 100 plates per case |
Storage & Handling
- Store in original packaging in a clean, dry environment away from direct sunlight and UV exposure
- Maintain storage temperature between 15°C and 30°C prior to use
- Handle plates by the edges to avoid contaminating well surfaces with skin oils and RNases
- Use powder-free gloves when handling plates for sensitive RNA applications
- Load samples using a multichannel pipette or automated liquid handler to maintain well-to-well consistency
- Seal plates immediately after sample loading to prevent evaporation — verify seal integrity before placing in the thermal cycler
- Centrifuge briefly at low speed after sealing to collect sample at the well base and remove air bubbles
- Verify plate is compatible with your specific instrument model before use — confirm half-skirt format is accepted
- Dispose of used plates per your institution's biosafety and waste disposal guidelines
Frequently Asked Questions
What is a half-skirted plate and why does it matter?
A half-skirted plate has two raised side walls — typically along the long sides of the plate — that extend below the well base. These partial skirt walls provide support for robotic grippers and plate handlers during automated loading, while keeping the overall plate height low enough to fit the block format of ABI and other instruments that cannot accommodate a fully skirted plate. The half-skirt balances automation compatibility with instrument fit.
Which ABI instruments are these plates compatible with?
These half-skirted plates are compatible with Applied Biosystems thermal cyclers and qPCR instruments including the ABI 7500, 7900HT, and QuantStudio series that accept half-skirt plate formats. Always verify the recommended plate format in your specific instrument's documentation before ordering in bulk, as plate requirements can vary between ABI instrument models.
Why is black print labeling only available for 0.2 mL clear wells?
The available SKU combinations reflect what is stocked from the manufacturer. Black print labeling is offered on the 0.2 mL clear variant only. All other color and volume combinations — including all 0.1 mL variants and white well plates — are available with molded labeling, which is permanently formed into the plate frame and provides durable identification regardless of handling conditions.
When should I choose clear vs white wells?
Clear wells are the standard choice for most qPCR applications and are compatible with SYBR Green, probe-based, and multiplex detection methods. White wells are preferred for applications requiring maximum fluorescence sensitivity — the white surface reflects emitted light toward the detector, improving signal-to-noise ratio in low-template copy number assays and probe-based applications.
Are Certificates of Analysis available?
Yes — Certificates of Analysis (COA) are available upon request. Each lot is certified DNase/RNase-free and endotoxin-free. Contact us with your lot number for documentation.
Frequently Bought Together
These other products might interest you