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Dulbecco's Phosphate Buffered Saline (DPBS), 1X

Dulbecco's Phosphate Buffered Saline (DPBS), 1X

Dulbecco’s Phosphate Buffered Saline (DPBS) is a sterile, ready-to-use 1X balanced salt solution for washing, rinsing, and diluting cells. Choose without calcium and magnesium for rinsing before trypsinization or cell dissociation, where divalent cations would work against detachment. Choose with calcium and magnesium when you want cells to stay attached and keep their junctions intact during washes.

Storage/Shipping: Ships ambient. Store at room temperature until use.

Upon Receipt: Inspect for clarity and store at room temperature. Do not use if cloudy or precipitated.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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$ 17.55 $ 17.55 (Tax excluded)

For Research Use Only
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DPBS Product Description

Dulbecco's Phosphate Buffered Saline (DPBS) is a sterile, ready-to-use 1X balanced salt solution for washing, rinsing, and diluting cells, and for transporting cells or tissue samples. It holds cells at physiological pH and osmolality during handling steps outside of growth medium.

DPBS without calcium and magnesium (KM-DPBS-1): for rinsing cells before trypsin or EDTA. Removing calcium, magnesium, and residual serum lets cells release from the surface and from each other, and keeps serum trypsin inhibitors from slowing dissociation.

DPBS with calcium and magnesium (KM-DPBS-2): for washes where cells should stay attached and keep their junctions intact, such as rinses between steps or before imaging.

Specifications

SpecificationKM-DPBS-1KM-DPBS-2
Concentration1X1X
Calcium / MagnesiumNoneContains CaCl₂ and MgCl₂
Phenol RedNoneNone
AppearanceClear, colorless solutionClear, colorless solution
SterilitySterile filtered (0.1 µm or 0.2 µm)

Catalog Information

Catalog NumberKM-DPBS-1KM-DPBS-2
Formulation1X, without calcium and magnesium1X, with calcium and magnesium
Format500 mL, liquid (bottle)
Product TypeBalanced Salt Solution (Cell Culture Reagent)
Preservative / StabilizersNone added

Storage & Handling

Shipping ConditionAmbient (room temperature)
Storage TemperatureRoom temperature
StabilityStable until the expiry date stated on the bottle label when stored correctly
Do NotDo not freeze. Do not use a bottle that is cloudy or shows visible precipitate; contact us if this occurs.

Safety

WARNING! This product is a laboratory reagent. Handle using standard aseptic technique and appropriate personal protective equipment (PPE). Follow your institution's chemical safety and handling policies.

For Research Use Only. Not for use in diagnostic, therapeutic, or clinical procedures. Not for human or animal consumption.

DPBS Frequently Asked Questions

What's the difference between DPBS and PBS?

DPBS is a specific, defined formulation based on Dulbecco's original recipe, with sodium and potassium salts and a phosphate buffer, offered with or without calcium and magnesium. "PBS" is a general term, and recipes vary between labs and suppliers, some without potassium or with different phosphate levels. For cell work, DPBS gives you a consistent, known composition.

Do I need DPBS with or without calcium and magnesium?

Use DPBS without calcium and magnesium (KM-DPBS-1) to rinse cells before trypsin or EDTA. Calcium and magnesium support cell adhesion and compete with EDTA, so leaving them behind slows dissociation. Use DPBS with calcium and magnesium (KM-DPBS-2) when you want cells to stay attached, such as rinses between steps or before imaging.

Does it contain phenol red?

No. Both formulations are phenol red-free, so they won't interfere with imaging or absorbance-based assays.

How should I store it?

Store at room temperature. Do not freeze. Freezing can cause salts to precipitate, especially in the calcium and magnesium formulation.

My DPBS with calcium and magnesium looks cloudy. Can I use it?

No. Cloudiness usually means calcium phosphate has precipitated, which changes the free calcium and magnesium in solution. Contact us for a replacement.

Can I hold cells in DPBS?

Only briefly, for washing and handling. DPBS has no nutrients, so cell viability drops if suspensions sit in it for more than a few hours.