Description
Key Features
Specifications
Storage & Handling
FAQs
Product Description
Sterile medical-grade polypropylene cryovials rated from -196°C to 121°C for vapor phase liquid nitrogen storage and repeated freeze-thaw applications. Available in 2.0 mL with internal or external thread, and 5.0 mL external thread only. The external thread design eliminates the O-ring from cap contact with the sample — reducing the possibility of contamination from rubber seal material. Internal thread vials include an O-ring for maximum leak protection.
Each vial features black graduation printing and a white writing area for direct sample labeling, a self-standing flat base for rack-free bench use, and is supplied sterile, DNase/RNase-free, and endotoxin-free. 50 vials per bag, 1,000 per case.
❄
-196°C to 121°C
LN2 vapor phase to autoclave compatible
🌡️
Freeze-Thaw Stable
Repeated freeze-thaw cycles without failure
✏
Writing Area
White writing area + black graduation printing
Thread Type Options
Internal Thread
Internal Thread
O-ring: Yes — maximum leak protection
Available size: 2.0 mL only
Best for: Applications requiring the most secure seal during LN2 vapor phase storage and repeated freeze-thaw
External Thread
External Thread
O-ring: No — cap has no O-ring
Available sizes: 2.0 mL and 5.0 mL
Best for: Reduced contamination risk — no O-ring material in contact with sample
Key Features & Benefits
- Medical-grade polypropylene — consistent structural integrity from -196°C vapor phase liquid nitrogen through autoclave sterilization at 121°C
- Wide working temperature range — -196°C to 121°C covers the full range from cryogenic biobanking to autoclave sterilization in a single tube format
- Vapor phase liquid nitrogen compatible — stable for long-term storage in LN2 vapor phase for biobanking, cell line preservation, and biological sample archiving
- Repeated freeze-thaw compatible — withstands multiple freeze-thaw cycles without cracking, deformation, or seal failure
- External thread option — no O-ring in cap reduces the possibility of contamination from O-ring material contact — available in 2.0 and 5.0 mL
- Internal thread option (2.0 mL) — O-ring sealed cap provides maximum leak protection for demanding cryogenic storage workflows
- Black graduation printing + white writing area — clear volume reference markings and a dedicated writing surface for permanent marker labeling on all sizes
- Self-standing flat base — stands upright without a rack for convenient filling, labeling, and processing at the bench
- Fits standard 9×9 (81-well) cryogenic boxes — compatible with existing biobank storage infrastructure
- Sterile, DNase/RNase-free, endotoxin-free — certified for sensitive biological sample storage and molecular biology applications
- Color-coded cap inserts available separately — five-color inserts for visual sample identification in storage boxes — see Cryovial Cap Inserts
⚠️ Single-use only — do not reuse cryovials between samples. Always store in vapor phase liquid nitrogen — do not submerge directly in liquid phase as trapped LN2 can cause explosive vaporization on thawing.
Specifications
| Specification | Detail |
|---|---|
| Material | Medical-grade polypropylene |
| Available Volumes | 2.0 mL | 5.0 mL |
| Thread Options | Internal thread (2.0 mL) | External thread (2.0 mL and 5.0 mL) |
| O-Ring | Internal thread = Yes | External thread = No |
| Working Temperature | ❄ -196°C to 121°C |
| LN2 Vapor Phase | ✓ Stable for vapor phase liquid nitrogen |
| Freeze-Thaw | Compatible with repeated freeze-thaw cycles |
| Base Design | Self-standing flat bottom |
| Labeling | White writing area + black graduation printing |
| Cryogenic Box Fit | Standard 9×9 (81-well) cryogenic storage boxes |
| Sterile | ✨ Yes — individually packaged sterile |
| Single-Use | ⚠️ Single-use recommended |
| RNase / DNase-free | ✓ Certified |
| Endotoxin-free | ✓ Certified |
| Quality System | Manufactured under ISO 13485 |
| Packaging | 50 vials per bag | 1,000 vials per case |
Storage & Handling
- Store unused vials in original packaging in a clean, dry environment away from direct sunlight
- Maintain storage temperature between 15°C and 30°C prior to use
- Wear cryogenic gloves and face shield when working with liquid nitrogen storage systems
- Fill vials leaving adequate headspace — do not overfill as liquid expands on freezing
- Ensure screw cap is fully and evenly tightened before placing in cryogenic storage
- For internal thread vials, verify O-ring is correctly seated before tightening cap
- Place vials in standard 9×9 cryogenic storage boxes — label boxes clearly with sample ID, date, and storage conditions
- Store in vapor phase of liquid nitrogen only — do not submerge vials directly in liquid nitrogen phase
- Single-use only — do not reuse vials between samples
- Thaw vials at 37°C water bath or at room temperature — never use open flame or boiling water
- Dispose of used vials per your institution's biosafety and cryogenic waste disposal guidelines
⚠️ Always store in vapor phase liquid nitrogen — NOT submerged in liquid phase. Liquid nitrogen trapped inside a sealed vial can cause rapid pressurization and explosive vaporization on thawing. Always wear appropriate cryogenic PPE including face shield and insulated gloves.
Frequently Asked Questions
What is the difference between internal and external thread cryovials?
Internal thread vials have cap threads inside the tube opening with an O-ring seal — providing maximum leak protection during cryogenic storage and freeze-thaw cycling. External thread vials have threads on the outside of the tube neck with no O-ring in the cap — eliminating O-ring material contact with the sample and reducing the possibility of contamination from rubber seal material. External thread is the preferred choice for sensitive biological samples, cell lines, and molecular biology specimens where minimizing foreign material contact is important.
Why is the working temperature range -196°C to 121°C?
Medical-grade polypropylene maintains structural integrity across this full range — from vapor phase liquid nitrogen at -196°C for long-term cryogenic biobanking, through standard freezer and refrigerator storage, up to 121°C which is the standard autoclave sterilization temperature. This means the same vial format can be autoclave-sterilized before use and then stored in LN2 vapor phase — a unique advantage for workflows requiring pre-sterilization of vials before filling with sensitive samples.
Why store in vapor phase rather than liquid phase LN2?
Storing vials submerged directly in liquid nitrogen creates a safety risk — liquid nitrogen can penetrate into vials through imperfect seals. When the vial is removed and begins to warm, the trapped LN2 vaporizes rapidly, expanding approximately 700-fold in volume, which can cause the vial to shatter or explode with significant force. Vapor phase storage at -150°C to -190°C maintains full cryogenic preservation conditions while eliminating this risk. Most current biobanking protocols specify vapor phase storage for this reason.
Are color-coded cap inserts available?
Yes — color-coded cap inserts are available separately in white, red, green, yellow, and blue. They embed directly into the cryovial cap for instant visual identification when scanning storage boxes. Compatible with all cryovial sizes. See the Cryovial Cap Inserts product page for details.
Are Certificates of Analysis available?
Yes — Certificates of Analysis (COA) are available upon request. Each lot is certified RNase-, DNase-, and endotoxin-free, manufactured under ISO 13485. Contact us with your lot number for documentation.
Description
Key Features
Specifications
Storage & Handling
FAQs
Product Description
Sterile medical-grade polypropylene cryovials rated from -196°C to 121°C for vapor phase liquid nitrogen storage and repeated freeze-thaw applications. Available in 2.0 mL with internal or external thread, and 5.0 mL external thread only. The external thread design eliminates the O-ring from cap contact with the sample — reducing the possibility of contamination from rubber seal material. Internal thread vials include an O-ring for maximum leak protection.
Each vial features black graduation printing and a white writing area for direct sample labeling, a self-standing flat base for rack-free bench use, and is supplied sterile, DNase/RNase-free, and endotoxin-free. 50 vials per bag, 1,000 per case.
❄
-196°C to 121°C
LN2 vapor phase to autoclave compatible
🌡️
Freeze-Thaw Stable
Repeated freeze-thaw cycles without failure
✏
Writing Area
White writing area + black graduation printing
Thread Type Options
Internal Thread
Internal Thread
O-ring: Yes — maximum leak protection
Available size: 2.0 mL only
Best for: Applications requiring the most secure seal during LN2 vapor phase storage and repeated freeze-thaw
External Thread
External Thread
O-ring: No — cap has no O-ring
Available sizes: 2.0 mL and 5.0 mL
Best for: Reduced contamination risk — no O-ring material in contact with sample
Key Features & Benefits
- Medical-grade polypropylene — consistent structural integrity from -196°C vapor phase liquid nitrogen through autoclave sterilization at 121°C
- Wide working temperature range — -196°C to 121°C covers the full range from cryogenic biobanking to autoclave sterilization in a single tube format
- Vapor phase liquid nitrogen compatible — stable for long-term storage in LN2 vapor phase for biobanking, cell line preservation, and biological sample archiving
- Repeated freeze-thaw compatible — withstands multiple freeze-thaw cycles without cracking, deformation, or seal failure
- External thread option — no O-ring in cap reduces the possibility of contamination from O-ring material contact — available in 2.0 and 5.0 mL
- Internal thread option (2.0 mL) — O-ring sealed cap provides maximum leak protection for demanding cryogenic storage workflows
- Black graduation printing + white writing area — clear volume reference markings and a dedicated writing surface for permanent marker labeling on all sizes
- Self-standing flat base — stands upright without a rack for convenient filling, labeling, and processing at the bench
- Fits standard 9×9 (81-well) cryogenic boxes — compatible with existing biobank storage infrastructure
- Sterile, DNase/RNase-free, endotoxin-free — certified for sensitive biological sample storage and molecular biology applications
- Color-coded cap inserts available separately — five-color inserts for visual sample identification in storage boxes — see Cryovial Cap Inserts
⚠️ Single-use only — do not reuse cryovials between samples. Always store in vapor phase liquid nitrogen — do not submerge directly in liquid phase as trapped LN2 can cause explosive vaporization on thawing.
Specifications
| Specification | Detail |
|---|---|
| Material | Medical-grade polypropylene |
| Available Volumes | 2.0 mL | 5.0 mL |
| Thread Options | Internal thread (2.0 mL) | External thread (2.0 mL and 5.0 mL) |
| O-Ring | Internal thread = Yes | External thread = No |
| Working Temperature | ❄ -196°C to 121°C |
| LN2 Vapor Phase | ✓ Stable for vapor phase liquid nitrogen |
| Freeze-Thaw | Compatible with repeated freeze-thaw cycles |
| Base Design | Self-standing flat bottom |
| Labeling | White writing area + black graduation printing |
| Cryogenic Box Fit | Standard 9×9 (81-well) cryogenic storage boxes |
| Sterile | ✨ Yes — individually packaged sterile |
| Single-Use | ⚠️ Single-use recommended |
| RNase / DNase-free | ✓ Certified |
| Endotoxin-free | ✓ Certified |
| Quality System | Manufactured under ISO 13485 |
| Packaging | 50 vials per bag | 1,000 vials per case |
Storage & Handling
- Store unused vials in original packaging in a clean, dry environment away from direct sunlight
- Maintain storage temperature between 15°C and 30°C prior to use
- Wear cryogenic gloves and face shield when working with liquid nitrogen storage systems
- Fill vials leaving adequate headspace — do not overfill as liquid expands on freezing
- Ensure screw cap is fully and evenly tightened before placing in cryogenic storage
- For internal thread vials, verify O-ring is correctly seated before tightening cap
- Place vials in standard 9×9 cryogenic storage boxes — label boxes clearly with sample ID, date, and storage conditions
- Store in vapor phase of liquid nitrogen only — do not submerge vials directly in liquid nitrogen phase
- Single-use only — do not reuse vials between samples
- Thaw vials at 37°C water bath or at room temperature — never use open flame or boiling water
- Dispose of used vials per your institution's biosafety and cryogenic waste disposal guidelines
⚠️ Always store in vapor phase liquid nitrogen — NOT submerged in liquid phase. Liquid nitrogen trapped inside a sealed vial can cause rapid pressurization and explosive vaporization on thawing. Always wear appropriate cryogenic PPE including face shield and insulated gloves.
Frequently Asked Questions
What is the difference between internal and external thread cryovials?
Internal thread vials have cap threads inside the tube opening with an O-ring seal — providing maximum leak protection during cryogenic storage and freeze-thaw cycling. External thread vials have threads on the outside of the tube neck with no O-ring in the cap — eliminating O-ring material contact with the sample and reducing the possibility of contamination from rubber seal material. External thread is the preferred choice for sensitive biological samples, cell lines, and molecular biology specimens where minimizing foreign material contact is important.
Why is the working temperature range -196°C to 121°C?
Medical-grade polypropylene maintains structural integrity across this full range — from vapor phase liquid nitrogen at -196°C for long-term cryogenic biobanking, through standard freezer and refrigerator storage, up to 121°C which is the standard autoclave sterilization temperature. This means the same vial format can be autoclave-sterilized before use and then stored in LN2 vapor phase — a unique advantage for workflows requiring pre-sterilization of vials before filling with sensitive samples.
Why store in vapor phase rather than liquid phase LN2?
Storing vials submerged directly in liquid nitrogen creates a safety risk — liquid nitrogen can penetrate into vials through imperfect seals. When the vial is removed and begins to warm, the trapped LN2 vaporizes rapidly, expanding approximately 700-fold in volume, which can cause the vial to shatter or explode with significant force. Vapor phase storage at -150°C to -190°C maintains full cryogenic preservation conditions while eliminating this risk. Most current biobanking protocols specify vapor phase storage for this reason.
Are color-coded cap inserts available?
Yes — color-coded cap inserts are available separately in white, red, green, yellow, and blue. They embed directly into the cryovial cap for instant visual identification when scanning storage boxes. Compatible with all cryovial sizes. See the Cryovial Cap Inserts product page for details.
Are Certificates of Analysis available?
Yes — Certificates of Analysis (COA) are available upon request. Each lot is certified RNase-, DNase-, and endotoxin-free, manufactured under ISO 13485. Contact us with your lot number for documentation.
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