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KCL-ENG-103
⚠ FOR RESEARCH USE ONLY — Not for diagnostic or therapeutic procedures
KCL-ENG-103 is a stable, clonal HEK293 cell line engineered to express full-length human CD19 (Gene ID: 930) via lentiviral transduction. Following puromycin selection and limiting dilution cloning, a high-expressing clone was isolated for consistent, uniform CD19 surface expression across passages.
CD19 is expressed as a full-length surface glycoprotein (95 kDa) without fusion tags, preserving native conformation for antibody recognition and functional assay work. Greater than 90% of viable cells express human CD19 by flow cytometry, with expression stable for a minimum of 15 passages under continuous puromycin selection.
This cell line is well-suited for CAR-T cell cytotoxicity assays, ADCC studies, antibody screening, and flow cytometry standards. All lots are quality-tested prior to release for CD19 expression, post-thaw viability, mycoplasma, and sterility.
DMEM supplemented with 10% heat-inactivated FBS and 1% Pen/Strep. For routine maintenance under selection, add Puromycin at 1.0 μg/mL. Allow cells to recover in selection-free medium for 48 hours after thawing before reintroducing Puromycin. During co-culture assays with immune cells, omit Puromycin — it is toxic to lymphocytes.
All lots are quality tested prior to release. The following characterization data is generated for each production lot.
| Parameter | Result | Specification |
|---|---|---|
| Human CD19 expression | ✓ Positive | ≥90% by flow cytometry; MFI >10-fold over isotype control |
| Expression stability | ✓ Stable | Stable for minimum 15 passages under Puromycin selection |
| Post-thaw viability | ✓ Pass | >80% by Trypan Blue exclusion |
| Transgene | ✓ Confirmed | Full-length human CD19 (Gene ID: 930); untagged surface glycoprotein; 95 kDa |
| Selection marker | ✓ Confirmed | Puromycin resistance; 1.0 μg/mL for maintenance |
| Parental cell line | ✓ Confirmed | HEK293 (ATCC CRL-1573); epithelial, adherent; ~24 hr doubling time |
| Test | Result |
|---|---|
| Mycoplasma (qPCR) | ✗ Negative |
| Sterility — Bacteria | ✗ Negative |
| Sterility — Fungi | ✗ Negative |
Certificates of Analysis with lot-specific QC data including flow cytometry histograms are available upon request. Contact Kulture Scientific with your lot number.
KCL-ENG-103 is shipped as a cryopreserved vial on dry ice. Follow the steps below upon receipt. Detailed subculture and cryopreservation protocols are available on the Kulture Scientific protocol page.
Subculture at 80 to 90% confluence using 0.25% Trypsin-EDTA. Split 1:3 to 1:5 every 2 to 3 days. Doubling time is approximately 24 hours. Maintain under Puromycin selection (1.0 μg/mL) to preserve stable CD19 expression. Full protocols are available on the Kulture Scientific protocol page.
KCL-ENG-103 is available as a cryopreserved vial containing at least 5×10&sup5; viable cells in cryoprotective medium containing DMSO.
| Product | Cat. No. | Format | Cell Number |
|---|---|---|---|
| HEK293 / Human CD19 Expression Stable Cell Line | KCL-ENG-103 | Cryopreserved Vial | ≥5×105 cells/vial |
- Storage — store in liquid nitrogen vapor phase (−150°C to −196°C); this is the only recommended storage condition for cryopreserved cells
- Do not store at −80°C or −20°C — neither temperature is suitable for long-term cell storage; ice crystal formation at these temperatures will irreversibly damage cell membranes and compromise viability
- Temperature sensitivity — minimize time outside cryogenic storage; cells are sensitive to temperature fluctuations
- Freeze-thaw cycles — repeated freeze-thaw cycles compromise cell viability and CD19 expression stability; thaw only the quantity needed for immediate use
- Stability — cells stored in liquid nitrogen are stable indefinitely when maintained under correct cryogenic conditions
- Shipping condition — cryopreserved vials are shipped on dry ice; inspect packaging upon receipt and transfer to liquid nitrogen storage immediately
- DMSO content — cryoprotective medium contains DMSO; wear nitrile gloves during thaw procedures and avoid skin contact
- Certificate of Analysis — lot-specific QC documentation including flow cytometry data, viability, and sterility results available upon request
For research purposes only. Not for use in diagnostic or therapeutic procedures. This product has not been tested or validated for clinical use. Not for direct administration into humans or animals.
This cell line should be handled under Biosafety Level 2 (BSL-2) conditions based on the following characteristics:
- Human-derived origin — all human-derived cell lines require BSL-2 containment under Universal Precautions regardless of donor screening status
- Lentiviral transduction history — the line was generated using lentiviral vectors; replication-incompetent lentivirally transduced lines are classified as BSL-2 by most institutional biosafety committees
- Stable transgene integration — integrated transgene expression warrants appropriate institutional review and containment
Users must consult their institutional biosafety committee (IBC) prior to use. Individual institutions may impose additional requirements based on local policy.
- Human-derived material — treat as potentially infectious; follow BSL-2 guidelines for handling human-derived cell lines
- Lentiviral transduction history — handle in accordance with your IBC guidelines for lentivirally transduced lines
- Puromycin — toxic to lymphocytes; omit from co-culture assays with immune cells; handle in accordance with institutional chemical safety guidelines
- DMSO (cryoprotectant) — skin-penetrating solvent; wear nitrile gloves at all times during thawing
- Disposal — decontaminate all cell culture waste with 10% bleach prior to disposal in accordance with local biohazardous waste regulations
⚠ FOR RESEARCH USE ONLY — Not for diagnostic or therapeutic procedures
KCL-ENG-103 is a stable, clonal HEK293 cell line engineered to express full-length human CD19 (Gene ID: 930) via lentiviral transduction. Following puromycin selection and limiting dilution cloning, a high-expressing clone was isolated for consistent, uniform CD19 surface expression across passages.
CD19 is expressed as a full-length surface glycoprotein (95 kDa) without fusion tags, preserving native conformation for antibody recognition and functional assay work. Greater than 90% of viable cells express human CD19 by flow cytometry, with expression stable for a minimum of 15 passages under continuous puromycin selection.
This cell line is well-suited for CAR-T cell cytotoxicity assays, ADCC studies, antibody screening, and flow cytometry standards. All lots are quality-tested prior to release for CD19 expression, post-thaw viability, mycoplasma, and sterility.
DMEM supplemented with 10% heat-inactivated FBS and 1% Pen/Strep. For routine maintenance under selection, add Puromycin at 1.0 μg/mL. Allow cells to recover in selection-free medium for 48 hours after thawing before reintroducing Puromycin. During co-culture assays with immune cells, omit Puromycin — it is toxic to lymphocytes.
All lots are quality tested prior to release. The following characterization data is generated for each production lot.
| Parameter | Result | Specification |
|---|---|---|
| Human CD19 expression | ✓ Positive | ≥90% by flow cytometry; MFI >10-fold over isotype control |
| Expression stability | ✓ Stable | Stable for minimum 15 passages under Puromycin selection |
| Post-thaw viability | ✓ Pass | >80% by Trypan Blue exclusion |
| Transgene | ✓ Confirmed | Full-length human CD19 (Gene ID: 930); untagged surface glycoprotein; 95 kDa |
| Selection marker | ✓ Confirmed | Puromycin resistance; 1.0 μg/mL for maintenance |
| Parental cell line | ✓ Confirmed | HEK293 (ATCC CRL-1573); epithelial, adherent; ~24 hr doubling time |
| Test | Result |
|---|---|
| Mycoplasma (qPCR) | ✗ Negative |
| Sterility — Bacteria | ✗ Negative |
| Sterility — Fungi | ✗ Negative |
Certificates of Analysis with lot-specific QC data including flow cytometry histograms are available upon request. Contact Kulture Scientific with your lot number.
KCL-ENG-103 is shipped as a cryopreserved vial on dry ice. Follow the steps below upon receipt. Detailed subculture and cryopreservation protocols are available on the Kulture Scientific protocol page.
Subculture at 80 to 90% confluence using 0.25% Trypsin-EDTA. Split 1:3 to 1:5 every 2 to 3 days. Doubling time is approximately 24 hours. Maintain under Puromycin selection (1.0 μg/mL) to preserve stable CD19 expression. Full protocols are available on the Kulture Scientific protocol page.
KCL-ENG-103 is available as a cryopreserved vial containing at least 5×10&sup5; viable cells in cryoprotective medium containing DMSO.
| Product | Cat. No. | Format | Cell Number |
|---|---|---|---|
| HEK293 / Human CD19 Expression Stable Cell Line | KCL-ENG-103 | Cryopreserved Vial | ≥5×105 cells/vial |
- Storage — store in liquid nitrogen vapor phase (−150°C to −196°C); this is the only recommended storage condition for cryopreserved cells
- Do not store at −80°C or −20°C — neither temperature is suitable for long-term cell storage; ice crystal formation at these temperatures will irreversibly damage cell membranes and compromise viability
- Temperature sensitivity — minimize time outside cryogenic storage; cells are sensitive to temperature fluctuations
- Freeze-thaw cycles — repeated freeze-thaw cycles compromise cell viability and CD19 expression stability; thaw only the quantity needed for immediate use
- Stability — cells stored in liquid nitrogen are stable indefinitely when maintained under correct cryogenic conditions
- Shipping condition — cryopreserved vials are shipped on dry ice; inspect packaging upon receipt and transfer to liquid nitrogen storage immediately
- DMSO content — cryoprotective medium contains DMSO; wear nitrile gloves during thaw procedures and avoid skin contact
- Certificate of Analysis — lot-specific QC documentation including flow cytometry data, viability, and sterility results available upon request
For research purposes only. Not for use in diagnostic or therapeutic procedures. This product has not been tested or validated for clinical use. Not for direct administration into humans or animals.
This cell line should be handled under Biosafety Level 2 (BSL-2) conditions based on the following characteristics:
- Human-derived origin — all human-derived cell lines require BSL-2 containment under Universal Precautions regardless of donor screening status
- Lentiviral transduction history — the line was generated using lentiviral vectors; replication-incompetent lentivirally transduced lines are classified as BSL-2 by most institutional biosafety committees
- Stable transgene integration — integrated transgene expression warrants appropriate institutional review and containment
Users must consult their institutional biosafety committee (IBC) prior to use. Individual institutions may impose additional requirements based on local policy.
- Human-derived material — treat as potentially infectious; follow BSL-2 guidelines for handling human-derived cell lines
- Lentiviral transduction history — handle in accordance with your IBC guidelines for lentivirally transduced lines
- Puromycin — toxic to lymphocytes; omit from co-culture assays with immune cells; handle in accordance with institutional chemical safety guidelines
- DMSO (cryoprotectant) — skin-penetrating solvent; wear nitrile gloves at all times during thawing
- Disposal — decontaminate all cell culture waste with 10% bleach prior to disposal in accordance with local biohazardous waste regulations
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